Borrelia Burgdorferi anticorps (FITC)

N° du produit ABIN236886

Cet anticorps anti-Borrelia Burgdorferi est un anticorps Lapin Polyclonal détectant Borrelia Burgdorferi dans WB, IF et IHC (p). Adapté pour Borrelia burgdorferi.

Aperçu rapide pour Borrelia Burgdorferi anticorps (FITC) (ABIN236886)

Antigène: Borrelia Burgdorferi

Reactivité: Borrelia burgdorferi

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Borrelia Burgdorferi est conjugé à/à la FITC

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Borrelia Burgdorferi anticorps (FITC)

Specificité: Reactive Western blot bands at 83kD, 41kD, 34kD and 31kD and additional low MW bands. Cross-reacts with Treponema pallidum, B. hermsii & B. parkerii.

Attributs du produit: Rabbit anti B. Burgdorferi, Rabbit antibody to Borrelia burgdorferi (Lyme) Fluorescein conjugated

Purification: Covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

Immunogène: Whole cell preparation from B. burgdorferi.

Information d'application

Indications d'application: Suitable for use in direct IFA, Western blot and immunohistochemistry (formalin fixed/paraffin). Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Protocol for direct IFA: 1. Multi-well slide contains acetone fixed organisms (BION Enterprises). 2. 2-fold dilutions of ABIN236886 starting at 1:10 out to 1:80 with a counterstain blocking diluents (Evan’s Blue in BSA solution). 3. Incubate 30 minutes at Room Temperature. 4. Wash 5. Mounting medium added to each well before coverslipping the slide. 6. Typically observe 4+ staining for several of the dilutions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 0.01 M PBS, pH7.2 containing 10 mg/mL BSA, 0.1 % Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid multiple freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.

Stock: 4 °C/-20 °C

Stockage commentaire: Store (up to 6 months) at 2-8 °C under subdued light. Long term, aliquot and store at -28 °C.

Date de péremption: 6 months

Détails sur Borrelia Burgdorferi

Antigène: Borrelia Burgdorferi

Autre désignation: Borrelia Burgdorferi (Lyme)